Tupelo is the county seat and the largest city of Lee County, Mississippi. It is also the seventh-largest city in the state. It is situated in northeast Mississippi, between Memphis, Tennessee, and Birmingham, Alabama, and is accessed by U.S. Route 78. As of the 2010 census, the population was 34,546, with the surrounding counties of Lee, Pontotoc and Itawamba supporting a population of 138,976.

The city is best known as the birthplace of Elvis Presley. As an infant in Tupelo in 1936, Presley survived a tornado that was ranked as the fourth deadliest in United States history and killed more than 230 people.